Applications

A well-established DNA barcoding system offers an automated, fast, reliable and cost-effective tool for species identification even for non-experts. All GBOL metadata (validated DNA barcodes, laboratory protocols, species lists, species distribution maps, etc.) will be made available in public databases (DNA sequence data currently mainly via BOLD). Free and unrestricted online access to GBOL data allows various applications such as:

• Biodiversity monitoring and environmental assessment
• Rapid assessment opportunity for planning and monitoring of conservation programs
• Species identification and species composition analyses of mixed environmental samples (e.g. soil, water, insect traps)
• Detection and monitoring of endangered and invasive species, pests and disease vectors
• Controlling illegal wildlife trade
• DNA-based diet analysis of scat samples
• Detection of food labeling fraud
• Trace analysis in forensics and criminology
• Discovery of new species
• Identification of larval stages in species (i.e. larvae, eggs and spores)

For some specific applications we will – together with partners from the practice – develop and test protocols and Standard Operating Procedures (SOPs) for new methodological approaches in the second phase:

1. Innovative, NGS-based tool to assess Water Framework Directive relevant diatoms
2. Verification of seed and nursery products
3. Barcoding pollination in agriculture
4. Identification of airborne pollen and pollen in honey
5. Fungal pathogens & necrotic fungi in orchards
6. Egg-parasitoids in Trichogramma for biological pest control
7. Innovative, NGS-based tool to assess Water Framework Directive relevant aquatic taxa from mixed samples
8. Environmental DNA from terrestrial mass samples and identification of forensically and medically important insect species

Innovative, NGS-based tool to assess Water Framework Directive relevant diatoms

BGBM, Freie Universität Berlin – Zentraleinrichtung Botanischer Garten und Botanisches Museum Berlin-Dahlem

Contact:

Dr. Regine Jahn (r.jahn@bgbm.org)

Team: Juliane Bettig (TA) Dr. Nelida Abarca (Wiss) Dr. Oliver Skibbe (Wiss) Katja Luther (Wiss) Ahn Van (SHK) Dr. Jonas Zimmermann (Wiss)

See also:

• Zimmermann J, Abarca N, Enk N, Skibbe O, Kusber WH, Jahn R (2014) Taxonomic Reference Libraries for Environmental Barcoding: A Best Practice Example from Diatom Research. PLoS ONE 9, e108793.
• Zimmermann J, Glöckner G, Jahn R, Enke N, Gemeinholzer B (2014) Metabarcoding vs. morphological identification to assess diatom diversity in environmental studies. Molecular Ecology Resources, 15(3), 526-542.
• Zimmermann J, Jahn R, Gemeinholzer B (2011) Barcoding diatoms: evaluation of the V4 subregion on the 18S rRNA gene, including new primers and protocols. Organisms Diversity & Evolution 11, 173‐192.

Verification of seed and nursery products

BGBM, Freie Universität Berlin – Zentraleinrichtung Botanischer Garten und Botanisches Museum Berlin-Dahlem

Rheinische Friedrich-Wilhelms-Universität Bonn, Nees-Institut für Biodiversität der Pflanzen

IEB, Westfälische Wilhelm-Universität Münster, AG Evolution und Biodiversität der Pflanzen

Contact:

Prof. Dr. Thomas Borsch (t.borsch@bgbm.org)

Prof. Dr. Dietmar Quandt (quandt@uni-bonn.de)

Prof. Dr. Kai Müller (kaimueller@uni-muenster.de)

Sarah Wiechers (sarah.wiechers@uni-muenster.de)

See also:

• http://gbol5.de/

Barcoding pollination in agriculture – recommendations for enhancement of pollinators, biodiversity and yield

INRES, Rheinische Friedrich-Wilhelms-Universität Bonn – Landwirtschaftliche Fakultät –

Institut für Nutzpflanzenwissenschaften und Ressourcenschutz – Agrar- und Produktionsökologie

ZFMK, Zoologisches Forschungsmuseum A. Koenig, Bonn

Contact:

Dr. Andreé Hamm (a.hamm@uni-bonn.de)

Msc Isabel Kilian (i.kilian@gmx.de)

Dr. Ralph Peters (r.peters@zfmk.de)

Dr. Ximo Mengual (x.mengual@zfmk.de)

Dipl.-Biol. Björn Rulik (b.rulik@zfmk.de)

See also:

• Ssymank A, Hamm A, Vischer-Leopold M (Eds.) (2009) Caring for Pollinators, Safeguarding Agro-biodiversity and Wild Plant Diversity. BfN-Skripten 250. Bonn, p. 191. http://www.bfn.de/fileadmin/MDB/documents/service/Skript250.pdf (accessed 25.01.16)

Identification of airborne pollen and pollen in honey

JLU, Justus Liebig Universität Giessen

TIEM (Team Integrierte Umweltüberwachung GbR)

Contact:

PD Dr. Birgit Gemeinholzer (birgit.gemeinholzer@bot1.bio.unigiessen)

Dipl.-Biol. Frieder Hofmann

Stephanie Swenson-Friedrich (Stephanie.Swenson-Friedrich@bot1.bio.uni-giessen.de)

See also:

• Vortrag zu GVO – Pollenmonitoring
• Keller, A., Danner, N., Grimmer, G., Ankenbrand, M., Ohe, K., Ohe, W., … & Steffan‐Dewenter, I. (2015). Evaluating multiplexed next‐generation sequencing as a method in palynology for mixed pollen samples. Plant Biology, 17(2), 558-566.

Fungal pathogens & necrotic fungi in orchards

RUB, Ruhr-Universität Bochum (Koordination)

TI, Thünen-Institut für Forstgenetik

SMNG, Senckenberg, Naturkundemuseum Görlitz

SMNS, Staatliches Museum für Naturkunde Stuttgart

Universität Bayreuth

SMNK, Staatliches Museum für Naturkunde Karlsruhe

HZI, Helmholtz-Zentrum für Infektionsforschung

Contact:

Prof. Dr. Dominik Begerow (dominik.begerow@rub.de)

Dr. Ben Bubner (ben.bubner@ti.bund.de)

Dr. Ulrike Damm (ulrike.damm@senckenberg.de)

Dr. Ursula Eberhardt (ursula.eberhardt@smns-bw.de)

Prof. Dr. Gerhard Rambold (gerhard.rambold@uni-bayreuth.de)

Dr. Markus Scholler (markus.scholler@smnk.de)

Prof. Dr. Marc Stadler (Marc.Stadler@helmholtz-hzi.de)

Janno Harjes (Janno.Harjes@uni-bayreuth.de) AG Mykologie, Universität Bayreuth Phone: +49 (0)921 55-2456

Steffen Bien (steffen.bien@senckenberg.de) Abteilung Botanik, Sektion Mykologie, Senckenberg Museum für Naturkunde Görlitz Phone: +49 (0)3581 4760 5314

See also:

• Eberhardt U, Verbeken A, Nuytinck,J (2014) DNA barcoding, Pilze und das German Barcode of Life-Projekt (GBOL). Zeitschrift für Mykologie 80: 627-641
• Eberhardt U (2012). Methods for DNA barcoding fungi. In: Kress, J.W., Erickson, D.L. (ed.) DNA Barcodes: Methods and Protocols. Humana Press, pp. 183-205
• Stielow JB, Lévesque CA, Seifert KA, Meyer W, Irinyi L, Smits D, Renfurm R, Verkley GJM, Groenewald M, Chaduli D, Lomascolo A, Welti S, Lesage-Meessen L, Favel A, Al-Hatmi AMS, Damm U, Yilmaz N, Houbraken J, Lombard L, Quaedvlieg W, Binder M, Vaas LAI, Vu D, Yurkov A, Begerow D, Roehl O, Guerreiro M, Fonseca A, Samerpitak K, van Diepeningen AD, Dolatabadi S, Moreno LF, Casaregola S, Mallet S, Jacques N, Roscini L, Egidi E, Bizet C, Garcia-Hermoso D, Martín MP, Deng S, Groenewald JZ, Boekhout T, de Beer ZW, Barnes I, Duong TA, Wingfield MJ, de Hoog GS, Crous PW, Lewis CT, Hambleton S, Moussa TAA, Al-Zahrani HS, Almaghrabi OA, Louis-Seize G, Assabgui R, McCormick W, Omer G, Dukik K, Cardinali G, Eberhardt U, de Vries M, Robert V. (2015) One fungus, which genes? Development and assessment of universal primers for potential secondary fungal DNA barcodes. Persoonia 35: 242-263 http://dx.doi.org/10.3767/003158515X689135

Egg-parasitoids in Trichogramma for biological pest control

SMNS, Staatliches Museum für Naturkunde Stuttgart

ZFMK, Zoologisches Forschungsmuseum A. Koenig, Bonn

Contact:

Dr. Lars Krogmann (lars.krogmann@smns-bw.de)

Dr. Ralph S. Peters (r.peters@zfmk.de)

See also:

• Samara, R., Monje, J.C., Zebitz, C.P.W. & Qubbaj, T. (2011) Comparative biology and life tables of Trichogramma aurosum on Cydia pomonella at constant temperatures. Phytoparasitica 39(2), 109-119
• Alkarrat, H. (2013) Inter- and intraspecific variation of nutritional and environmental adaptation of egg-parasitoids of the genus Trichogramma (Hymenoptera, Trichogrammatidae). PhD Thesis

Innovative, NGS-based tool to assess Water Framework Directive relevant aquatic taxa from mixed samples

UDE, Universität Duisburg-Essen

ZFMK, Zoologisches Forschungsmuseum A. Koenig, Bonn

Contact:

Prof. Dr. Florian Leese (florian.leese@uni-due.de)

Dr. Matthias Geiger (m.geiger@zfmk.de)

Vera Zizka (vera.zizka@uni-due.de) Fakultät für Biologie, Aquatische Ökosystemforschung

See also:

• Elbrecht V & Leese F (2015) Can DNA-based ecosystem assessments quantify species abundance? Testing primer bias and biomass – sequence relationships with an innovative metabarcoding protocol. PLOS ONE 10:e0130324
• Macher J-N, Salis RK, Blakemore K, Tollrian R, Matthaei C & Leese F (2015) Multiple-stressor effects on stream invertebrates: DNA barcoding reveals contrasting responses of cryptic mayfly species. Ecological Indicators 61(2): 159-169

Environmental DNA from terrestrial mass samples and identification of forensically and medically important insect species

ZSM, Generaldirektion der Staatlichen Naturwissenschaftlichen Sammlungen Bayerns – Zoologische Staatssammlung München

Contact:

Dr. Axel Hausmann (Axel.Hausmann@zsm.mwn.de)

Dipl.-Biol. Jerome Moriniere (moriniere@zsm.mwn.de)

See also:

• Interim report GBOL phase I (2014)